Cell Signaling Technology

Product Pathways - Development

Non-phospho-β-Catenin (Ser33/37/Thr41) Antibody #4270

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk (C) (X) (Z) (Dg) Endogenous 92 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  X=Xenopus  Z=Zebrafish  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Non-phospho-β-catenin (Ser33/37/Thr41) Antibody detects endogenous levels of β-catenin when residues Ser33, Ser37 and Thr41 are not phosphorylated.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser37 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of SW480 cells treated with Calyculin A #9902 and immunoprecipitated using Phospho-β-Catenin (Ser33/37) Antibody #2009 (Lane 2), β-Catenin Antibody (Amino-terminal Antigen) #9581 (Lane 3) and an unrelated rabbit IgG as negative control (Lane 4). The immunoprecipitates along with the input lysate (Lane 1) were subject to western analysis using β-Catenin (6B3) Rabbit mAb #9582 (upper), Phospho-β-Catenin (Ser33/37) Antibody #2009 (middle), and Non-phospho-β-catenin (Ser33/37/Thr41) Antibody (lower).

Western Blotting

Western Blotting

Western blot analysis of total SW480 cell lysates using Non-phospho-β-Catenin (Ser33/Ser37/Thr41) Antibody in the presence of a non-phospho-β-catenin peptide (Lane 4) and various peptides phosphorylated at different positions, including phospho-β-catenin (Ser33) peptide (Lane 1), phospho-β-catenin (Ser33/37) peptide (Lane 2), phospho-β-catenin (Ser33/37/Thr41) peptide (Lane 3), phospho-β-catenin (Ser37) peptide (Lane 5), phospho-β-catenin (Ser37/Thr41) peptide (Lane 6), and phospho-β-catenin (Thr41) peptide (Lane 7).

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
  2. Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
  3. Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
  5. Lin, C. et al. (2002) Cell 108, 837-847.
  6. Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
  7. Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
  8. Morin, P.J. (1997) Science 275, 1787-1790.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products