Product Pathways - Apoptosis / Autophagy
Cytochrome c Antibody #4272
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P | H M R Mk (B) | Endogenous | 14 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Cytochrome c Antibody detects endogenous levels of cytochrome c protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acids 67-78 of human cytochrome c. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western analysis of cell extracts from human (HeLa and Jurkat), monkey (COS), mouse (L929), rat (C6) and purified bovine cytochrome c, using Cytochrome c Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Cytochrome c Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Cytochrome c Antibody.
Background
Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).
- Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159.
- Li, P. et al. (1997) Cell 91, 479-489 .
- Liu, X. et al. (1996) Cell 86, 147-157.
Application References
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Companion Products
- 4280 Cytochrome c (136F3) Rabbit mAb
- 1033 Cytochrome c Blocking Peptide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
