Cell Signaling Technology

Product Pathways - Apoptosis

Cytochrome c Antibody #4272

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P H M R Mk Dm (B) Endogenous 14 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dm=D. melanogaster  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Cytochrome c Antibody detects endogenous levels of cytochrome c protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids 67-78 of human cytochrome c. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western analysis of cell extracts from human (HeLa and Jurkat), monkey (COS), mouse (L929), rat (C6) and purified bovine cytochrome c, using Cytochrome c Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Cytochrome c Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Cytochrome c Antibody.


Background

Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).

  1. Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159.
  2. Li, P. et al. (1997) Cell 91, 479-489 .
  3. Liu, X. et al. (1996) Cell 86, 147-157.

Application References

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Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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