Cell Signaling Technology

Product Pathways - Translational Control

ATP2A1/SERCA1 (A988) Antibody #4274

Applications Reactivity Sensitivity MW (kDa) Source
W M R Endogenous 100 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ATP2A1/SERCA1 (A988) Antibody detects endogenous levels of total ATP2A1/SERCA1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala988 of mouse ATP2A1/SERCA1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of mouse skeletal muscle extracts using ATP2A1/SERCA1 (A988) Antibody.

Background

Sarcoplasmic and endoplasmic reticulum Ca2+ ATPases (SERCA) are members of a highly conserved family of Ca2+ pumps (1). SERCA pumps transport Ca2+ from the cytosol to the sarcoplasmic and endoplasmic reticulum lumen against a large concentration gradient (1). ATP2A1 (SERCA1) is a fast-twitch, skeletal muscle sarcoplasmic reticulum Ca2+ ATPase (2). Research studies have shown that mutations in the ATP2A1 gene cause an autosomal recessive muscle disorder known as Brody myopathy, which is characterized by muscle cramping and impaired muscle relaxation associated with exercise (1-3).

  1. Hovnanian, A. (2007) Subcell Biochem 45, 337-63.
  2. Odermatt, A. et al. (1996) Nat Genet 14, 191-4.
  3. Odermatt, A. et al. (2000) Hum Genet 106, 482-91.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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