Product Pathways - Cytoskeletal Signaling
Pan-Keratin (C11) Mouse mAb (Biotinylated) #4279
|4279S||100 µl (10 western blots)||---||In Stock||---|
|4279||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Rat, Monkey||Endogenous||46-58||Mouse IgG1|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: W=Western Blotting, F=Flow Cytometry
Specificity / Sensitivity
Pan-Keratin (C11) Mouse mAb (Biotinylated) detects endogenous levels of total keratin 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a cytoskeleton preparation from A-431 cells.
Western blot analysis of extracts from HeLa and H-4-II-E cells using Pan-Keratin (C11) Mouse mAb (Biotinylated) detected using Streptavidin-HRP #3999.
This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated Pan-Keratin (C11) Mouse mAb #4545 reacts with human, rat and monkey pan-keratin. CST expects that Pan-Keratin (C11) Mouse mAb (Biotinylated) will also recognize pan-keratin in these species.
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 3999 Streptavidin-HRP
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- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 4548 Keratin 18 (DC10) Mouse mAb
- 4523 Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 4528 Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.