Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

Cytochrome c (136F3) Rabbit mAb #4280

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P H M R Mk Endogenous 14 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Cytochrome c (136F3) Rabbit mAb detects endogenous levels of total cytochrome c protein.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acids 67-78 of human cytochrome c.

Western Blotting

Western Blotting

Western blot analysis of cell lysates from various cell lines, using Cytochrome c (136F3) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Cytochrome c (136F3) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor, using Cytochrome c (136F3) Rabbit mAb #4280.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Cytochrome c (136F3) Rabbit mAb in the presence of control peptide (left) or Cytochrome c Blocking Peptide #1033 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Cytochrome c (136F3) Rabbit mAb .

Background

Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).

  1. Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159.
  2. Li, P. et al. (1997) Cell 91, 479-489 .
  3. Liu, X. et al. (1996) Cell 86, 147-157.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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