Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-Myt1 (Ser83) Antibody #4281

Applications Reactivity MW (kDa) Source
W H 70 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Myt1 (Ser83) Antibody detects endogenous levels of Myt1 only when phosphorylated at serine 83.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser83 of human Myt1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 cells, untreated or nocodazole-treated (50ng/ml), using Phospho-Myt1 (Ser83) Antibody (upper) or Myt1 Antibody #4282 (lower).

Background

Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-8).

Although Akt has been shown to phosphorylate Asterina (starfish) Myt1 at a consensus Akt phosphorylation site (7), the orthologous site, Ser83, in human Myt1 may be phosphorylated by a different kinase.

  1. Watanabe, N. et al. (1995) EMBO J. 14, 1878-1891.
  2. Hunter, T. (1995) Cell 80, 225-236.
  3. Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.
  4. McGowan, C.H. and Russell, P. (1993) EMBO J. 12, 75-85.
  5. Booher, R. N. et al. (1997) J. Biol. Chem. 272, 22300-22306.
  6. Palmer, A. et al. (1998) EMBO J. 17, 5037-5047.
  7. Okumura, E. et al. (2002) Nat. Cell Biol. 4, 111-116.
  8. Nakajima, H. et al. (2003) J. Biol. Chem. 278, 25277-25280.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Product Pathways

Drug Discovery Tools

Featured Technologies

Protein Classes