Product Pathways - Cytoskeletal Signaling
α-Tubulin (11H10) Rabbit mAb (Biotinylated) #4285
|W||H M R Mk Dm B (Dg)||Endogenous||52||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster B=Bovine Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
α-Tubulin (11H10) Rabbit mAb (Biotinylated) detects endogenous levels of total α-tubulin protein and does not cross-react with recombinant β-tubulin.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human α-tubulin.
This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (11H10) Rabbit mAb #2125.
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
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- 3999 Streptavidin-HRP
- 2125 α-Tubulin (11H10) Rabbit mAb
- 2128 β-Tubulin (9F3) Rabbit mAb
- 9099 α-Tubulin (11H10) Rabbit mAb (HRP Conjugate)
- 5346 β-Tubulin (9F3) Rabbit mAb (HRP Conjugate)
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7727 Biotinylated Protein Ladder Detection Pack
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.