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Oct-4 (9B7) Mouse mAb #4286

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP	H M	Endogenous	45	Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

4286:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Oct-4 (9B7) Mouse mAb detects endogenous levels of total Oct-4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human Oct-4 protein.

Background

Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).

1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278.
3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
4. Cheng, L. et al. (2007) J Pathol 211, 1-9.
5. Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20.
6. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
7. Lee, J. et al. (2006) J Biol Chem 281, 33554-65.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 7076 Anti-mouse IgG, HRP-linked Antibody
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 2750 Oct-4 Antibody
• 2840 Oct-4A (C30A3) Rabbit mAb
• 2890 Oct-4A (C52G3) Rabbit mAb

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For Research Use Only. Not For Use In Diagnostic Procedures.