Product Pathways - PI3K / Akt Signaling
PI3 Kinase p85 Antibody #4292
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 85 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
PI3 Kinase p85 Antibody detects endogenous levels of PI3 Kinase p85.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human PI3 Kinase p85. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).
- Cantley, L.C. (2002) Science 296, 1655-7.
- Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
- Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
- Stoyanov, B. et al. (1995) Science 269, 690-3.
Application References
- Sarfstein, R. et al. (2011) PLoS One 6, e24468. Applications: Western Blotting
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Companion Products
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- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 9901 LY294002 (PI3 Kinase Inhibitor)
- 9916 Phospho-Akt Pathway Antibody Sampler Kit
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.