Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

CHFR Antibody #4297

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 80 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CHFR Antibody detects endogenous levels of total CHFR protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence around Lys645 of human CHFR protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CHFR Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from MDA-MB-435 cells, untreated or nocodazole-treated, using CHFR Antibody.

Background

Checkpoint with forkhead and RING finger domains protein (CHFR) is an E3 ubiquitin-protein ligase that regulates cell cycle progression. In response to microtubule stress, CHFR delays the transition into mitosis by excluding cyclin B1 from the nucleus prior to chromosome condensation (1). Marked reduction of CHFR expression was detected in primary tumors and decreased CHFR expression was linked to promoter hypermethylation (1-4). Restoration of CHFR expression by treatment with the microtubule stress agent nocodazole and the methyltransferase inhibitor 5-aza-2'-deoxycytidine has been reported (4,5).

  1. Summers, M.K. et al. (2005) Oncogene 24, 2589-98.
  2. Privette, L.M. et al. (2007) Cancer Res 67, 6064-74.
  3. Morioka, Y. et al. (2006) Anticancer Res 26, 4267-70.
  4. Satoh, A. et al. (2003) Cancer Res 63, 8606-13.
  5. Dang, C. and Hudis, C. (2006) Clin Breast Cancer 7, 51-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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