Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

PDAP1 Antibody #4300

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 25 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PDAP1 Antibody detects endogenous levels of total PDAP1 protein. Nonspecific bands are seen above 60 KDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the center of human PDAP1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from L6, HeLa and NIH/3T3 cells using PDAP1 Antibody.

Background

Platelet derived growth factor (PDGF) proteins function as dimeric isoforms (i.e., PDGF AA, PDGF AB, PDGF BB, PDGF CC and PDGF DD) that bind receptor tyrosine kinases and activate cytoplasmic SH2 domain-containing proteins to control multiple signaling pathways that regulate angiogenesis, cell growth, actin reorganization, migration and differentiation (1). PDGFA-associated protein 1 (PDAP1) was originally identified as a novel, PDGF-associated protein found in a rat retinal tumor cell line (2). While copurified with PDGFA, PDAP1 interacts with PDGFB at a slightly higher affinity than with PDGFA (2). Although the exact function of PDAP1 is unclear, it has been shown to both increase PDGFA-induced incorporation of [3H]thymidine in Swiss 3T3 cells and decrease PDGFB growth factor activity (2). Ubiquitously expressed PDAP1 is highly conserved among species (2,3) and is phosphorylated in vitro by several kinases, including PKC, PKA, CKI and CKII. Among this group, CKII seems to be the major kinase that phosphorylates PDAP1 in intact cells (3).

  1. Ostman, A. and Heldin, C.H. (2001) Adv Cancer Res 80, 1-38.
  2. Fischer, W.H. and Schubert, D. (1996) J Neurochem 66, 2213-6.
  3. Shen, L. et al. (1996) Arch Biochem Biophys 327, 131-41.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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