Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Tyrosine Kinase / Adaptors

Mer (D21F11) XP® Rabbit mAb #4319

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H Endogenous 210 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Mer (D21F11) XP® Rabbit mAb detects endogenous levels of total Mer protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His925 of human Mer protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-MEL-5, Hep G2 and Jurkat cells using Mer (D21F11) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of SK-MEL-5 cells (left), Hep G2 cells (center) and MCF7 cells (right) using Mer (D21F11) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ® #4084 (fluorescent DNA dye).

Background

Mer tyrosine kinase belongs to a receptor tyrosine kinase family with Axl and Tyro3. This family is characterized by a common NCAM (neural adhesion molecule)-related extracellular domain and a common ligand, GAS6 (growth arrest-specific protein 6). Mer protein has an apparent molecular weight of 170-210 kDa due to different glycosylation patterns generated in different cell types. Mer can be activated by dimerization and autophosphorylation through ligand binding or homophilic cell-cell interaction mediated by its NCAM-like motif (1). The downstream signaling components of activated Mer include PI3 kinase, PLCγ, and MAP kinase (2). Family members are prone to transcriptional regulation and carry out diverse functions including the regulation of cell adhesion, migration, phagocytosis, and survival (3). Mer regulates macrophage activation, promotes apoptotic cell engulfment, and supports platelet aggregation and clot stability in vivo (4). Investigators ahve found that overexpression of Mer may play a cooperative role in leukemogenesis and may be an effective target for biologically based leukemia/lymphoma therapy (5).

  1. Ling, L. et al. (1996) J. Biol. Chem. 271, 18355-18362.
  2. Ling, L. and Kung, H.J. (1995) Mol. Cell Biol. 15, 6582-6592.
  3. Hafizi, S. and Dahlbäck, B. (2006) Cytokine Growth Factor Rev. 17, 295-304.
  4. Sather, S. et al. (2007) Blood 109, 1026-1033.
  5. Keating, A.K. et al. (2006) Oncogene 25, 6092-6100.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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