Product Pathways - Jak/Stat Pathway
Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4323
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| F | H M | Endogenous | 86kDa | Rabbit | IgG |
Applications Key:
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr705 of mouse Stat3. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-5.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (blue) or IFN-alpha treated (green), using Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb (Alexa Fluor® 488 Conjugate) compared to isotype control (red) #4340.
Description
Cell Signaling Technology Antibody conjugated to Alexa Fluor ® 488 fluorescent dye and tested in-house for direct Flow Cytometric analysis of human cells. The unconjugated antibody, #9145 reacts with Phospho- Stat3 (Tyr705) from human and mouse. CST expects that Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize Phospho-Stat3 in these species.
Background
The Stat3 transcription factor is an important signaling molecule for many cytokines and growth-factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
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- Biethahn, S. et al. (1999) Exp. Hematol. 27, 885-894.
Application References
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Companion Products
- 9131 Phospho-Stat3 (Tyr705) Antibody
- 4340 Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate)
- 9139 Stat3 (124H6) Mouse mAb
- 9138 Phospho-Stat3 (Tyr705) (3E2) Mouse mAb
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
