Cell Signaling Technology

Product Pathways - Neuroscience

Synaptophysin Antibody #4329

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 38 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Synaptophysin Antibody detects endogenous levels of total synaptophysin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala230 of human synaptophysin. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain, mouse brain and human cerebellum using Synaptophysin Antibody.

Background

Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).

  1. Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4.
  2. Valtorta, F. et al. (2004) Bioessays 26, 445-53.
  3. Arthur, C.P. and Stowell, M.H. (2007) Structure 15, 707-14.
  4. Bonanomi, D. et al. (2007) Biochem J 404, 525-34.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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