Cell Signaling Technology

Product Pathways - Protein Stability

UbcH5C (D60E2) Rabbit mAb #4330

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 14 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

UbcH5C (D60E2) Rabbit mAb detects endogenous levels of total UbcH5C protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp135 of human UbcH5C protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UbcH5C (D60E2) Rabbit mAb.

Background

Protein ubiquitination requires the concerted action of the E1, E2 and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through an ATP-dependent formation of a thiol ester with an E1 enzyme. The activated ubiquitin is then transferred to a thiol-group of an E2 ubiquitin-conjugation enzyme. The final step is the transfer of ubiquitin from E2 to an ε-amino group of a lysine residue on the target protein, a transfer mediated by ubiquitin-conjugating enzyme E3 (1). UbcH5C is a universally expressed E2 ubiquitin conjugating enzyme and member of the UbcH5 family that also includes UbcH5A and UbcH5B (2). Evidence suggests that UbcH5C plays an important role in regulating a number of signaling pathways by catalyzing the ubiquitination of key target proteins, including p53, PCNA, the IκB kinase protein NEMO, and the apoptosis inhibitor BRUCE (3-6). Gene expression profiles revealed increased expression of UbcH5C in meibomian cell carcinoma and oncocytic thyroid adenomas (7,8), while an RNAi screen reveals diffrential Ubc5HC in acute promyelocytic cells (9). These results suggest a potential role of UbcH5C in cell cycle control and tumorigenesis.

  1. Hershko, A. (1988) J Biol Chem 263, 15237-40.
  2. Jensen, J.P. et al. (1995) J Biol Chem 270, 30408-14.
  3. Saville, M.K. et al. (2004) J Biol Chem 279, 42169-81.
  4. Zhang, S. et al. (2008) Cell Cycle 7, 3399-404.
  5. Tang, E.D. et al. (2003) J Biol Chem 278, 37297-305.
  6. Qiu, X.B. et al. (2004) EMBO J 23, 800-10.
  7. Hattori, H. et al. (2007) Blood 110, 640-50.
  8. Kumar, A. et al. (2007) Genomics 90, 559-66.
  9. Jacques, C. et al. (2005) J Clin Endocrinol Metab 90, 2314-20.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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