Cell Signaling Technology

Product Pathways - Metabolism

Phospho-ATP-Citrate Lyase (Ser455) Antibody #4331

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M (R) Endogenous 125 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-ATP-Citrate Lyase (Ser455) Antibody detects endogenous levels of ATP-citrate lyase only when phosphorylated at Ser455 (equivalent to Ser454 in mouse).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser455 of human ATP-citrate lyase. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated for the indicated times, using Phospho-ATP-Citrate Lyase (Ser454) Antibody (upper) or ATP-Citrate Lyase Antibody #4332 (lower).

Background

ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholestrol and acetylcholine, as well as for glucogenesis (1). Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase (1,2). It is phosphorylated by GSK-3 on Thr446 and Ser450 (3). Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt (4,5). Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (2).

  1. Towle, H. C. et al. (1997) Annu. Rev. Nutr. 17, 405-433.
  2. Potapova, I. A. et al. (2000) Biochemistry 39, 1169-1179.
  3. Hughes , K. et al. (1992) Biochem. J. 288, 309-314.
  4. Pierce, M. W. et al. (1982) J. Biol. Chem. 257, 10681-10686.
  5. Berwick, D. C. et al. (2002) J. Biol.Chem. 277, 33895-33900.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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