Product Pathways - Glucose Metabolism
Phospho-ATP-Citrate Lyase (Ser454) Antibody #4331
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M (R) | Endogenous | 125 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-ATP-Citrate lyase (Ser454) Antibody detects endogenous levels of ATP-citrate lyase only when phosphorylated at serine 454.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser454 of human ATP-citrate lyase. Antibodies are purified by protein A and peptide affinity chromatography.
Background
ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholestrol and acetylcholine, as well as for glucogenesis (1). Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase (1,2). It is phosphorylated by GSK-3 on Thr446 and Ser450 (3). Ser454 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt (4,5). Phosphorylation on Ser454 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (2).
- Towle, H. C. et al. (1997) Annu. Rev. Nutr. 17, 405-433.
- Potapova, I. A. et al. (2000) Biochemistry 39, 1169-1179.
- Hughes , K. et al. (1992) Biochem. J. 288, 309-314.
- Pierce, M. W. et al. (1982) J. Biol. Chem. 257, 10681-10686.
- Berwick, D. C. et al. (2002) J. Biol.Chem. 277, 33895-33900.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.