Product Pathways - Glucose Metabolism
Phospho-ATP-Citrate Lyase (Ser454) Antibody #4331
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M (R) | Endogenous | 125 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-ATP-Citrate lyase (Ser454) Antibody detects endogenous levels of ATP-citrate lyase only when phosphorylated at serine 454.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser454 of human ATP-citrate lyase. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated for the indicated times, using Phospho-ATP-Citrate Lyase (Ser454) Antibody (upper) or ATP-Citrate Lyase Antibody #4332 (lower).
Background
ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholestrol and acetylcholine, as well as for glucogenesis (1). Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase (1,2). It is phosphorylated by GSK-3 on Thr446 and Ser450 (3). Ser454 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt (4,5). Phosphorylation on Ser454 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (2).
- Towle, H. C. et al. (1997) Annu. Rev. Nutr. 17, 405-433.
- Potapova, I. A. et al. (2000) Biochemistry 39, 1169-1179.
- Hughes , K. et al. (1992) Biochem. J. 288, 309-314.
- Pierce, M. W. et al. (1982) J. Biol. Chem. 257, 10681-10686.
- Berwick, D. C. et al. (2002) J. Biol.Chem. 277, 33895-33900.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4332 ATP-Citrate Lyase Antibody
- 9271 Phospho-Akt (Ser473) Antibody
- 9272 Akt Antibody
- 9275 Phospho-Akt (Thr308) Antibody
- 9840 Akt Kinase Assay Kit (Nonradioactive)
- 4051 Phospho-Akt (Ser473) (587F11) Mouse mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9331 Phospho-GSK-3α/β (Ser21/9) Antibody
- 9336 Phospho-GSK-3β (Ser9) Antibody
- 9621 Phospho-(Ser/Thr) PKA Substrate Antibody
- 9619 Immobilized Phospho-(Ser/Thr) Akt Substrate Antibody
- 9611 Phospho-(Ser/Thr) Akt Substrate Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
