Product Pathways - Metabolism
Phospho-ATP-Citrate Lyase (Ser455) Antibody #4331
|W IP||H M (R)||Endogenous||125||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-ATP-Citrate Lyase (Ser455) Antibody detects endogenous levels of ATP-citrate lyase only when phosphorylated at Ser455 (equivalent to Ser454 in mouse).
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser455 of human ATP-citrate lyase. Antibodies are purified by protein A and peptide affinity chromatography.
ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholestrol and acetylcholine, as well as for glucogenesis (1). Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase (1,2). It is phosphorylated by GSK-3 on Thr446 and Ser450 (3). Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt (4,5). Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (2).
- Towle, H. C. et al. (1997) Annu. Rev. Nutr. 17, 405-433.
- Potapova, I. A. et al. (2000) Biochemistry 39, 1169-1179.
- Hughes , K. et al. (1992) Biochem. J. 288, 309-314.
- Pierce, M. W. et al. (1982) J. Biol. Chem. 257, 10681-10686.
- Berwick, D. C. et al. (2002) J. Biol.Chem. 277, 33895-33900.
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For Research Use Only. Not For Use In Diagnostic Procedures.