Product Pathways - Glucose Metabolism
ATP-Citrate Lyase Antibody #4332
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 125 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
ATP-Citrate Lyase Antibody detects endogenous levels of total ATP-citrate lyase.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues of human ATP-citrate lyase. Antibodies are purified by protein A and peptide affinity chromatography
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using ATP-Citrate Lyase Antibody.
Background
ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholestrol and acetylcholine, as well as for glucogenesis (1). Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase (1,2). It is phosphorylated by GSK-3 on Thr446 and Ser450 (3). Ser454 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt (4,5). Phosphorylation on Ser454 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (2).
- Towle, H. C. et al. (1997) Annu. Rev. Nutr. 17, 405-433.
- Potapova, I. A. et al. (2000) Biochemistry 39, 1169-1179.
- Hughes , K. et al. (1992) Biochem. J. 288, 309-314.
- Pierce, M. W. et al. (1982) J. Biol. Chem. 257, 10681-10686.
- Berwick, D. C. et al. (2002) J. Biol.Chem. 277, 33895-33900.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
