Product Pathways - TGF-beta/Smad Signaling
Endoglin (D50G1) Rabbit mAb #4335
PhosphoSitePlus® protein, site, and accession data: ENG
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H | Endogenous | 95 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4335:
- Western Blotting
Specificity / Sensitivity
Endoglin (D50G1) Rabbit mAb detects endogenous levels of total endoglin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala463 of human endoglin protein.
Western Blotting
Western blot analysis of extracts from HUVEC cells using Endoglin (D50G1) Rabbit mAb.
Background
Endoglin (ENG, CD105) is an auxiliary receptor for the TGF-β receptor complex, functioning in related signaling pathways (1,2). Endoglin is a transmembrane protein that exists as a disulfide-linked homodimer. It is mainly expressed in vascular and connective tissues and in endothelial and stromal cells. Upregulated endoglin expression has been reported during wound healing and tumor vascularization, and in inflammatory tissues and developing embryos (1-4). Mutations in endoglin have been found to be a causal factor of hereditary hemorrhagic telangiectasia (HHT), a disease characterized by malformation of vascular structure (5,6). The importance of this protein for normal and tumor vascular function makes it a good marker for endothelial cell proliferation as well as a potential therapeutic target in cancer (4-6).
- Bernabeu, C. et al. (2007) J Cell Biochem 102, 1375-88.
- Zhu, Y. et al. (2003) Stroke 34, 2483-8.
- Quintanilla, M. et al. (2003) Oncogene 22, 5976-85.
- Dallas, N.A. et al. (2008) Clin Cancer Res 14, 1931-7.
- ten Dijke, P. et al. (2008) Angiogenesis 11, 79-89.
- Fernández-L, A. et al. (2006) Clin Med Res 4, 66-78.
Application References
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Companion Products
- 3290 Endoglin Antibody (Mouse Specific)
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
