Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Lymphocyte Signaling

AML1 (D33G6) XP® Rabbit mAb #4336

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P IF-IC F H Mk Endogenous 55 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

AML1 (D33G6) XP® Rabbit mAb detects endogenous levels of total AML1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids at the amino terminus of human AML1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using AML1 (D33G6) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using AML1 (D33G6) XP® Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using AML1 (D33G6) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of Jurkat cells using AML1 (D33G6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Background

AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).

  1. Wang, S. et al. (1993) Mol Cell Biol 13, 3324-3339.
  2. Ogawa, E. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 6859-6863.
  3. Okuda, T. et al. (1996) Cell 84, 321-30.
  4. Wang, Q. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 3444-3449.
  5. North, T.E. et al. (2004) Stem Cells 22, 158-168.
  6. Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-387.
  7. Tanaka, T. et al. (1996) Mol Cell Biol 16, 3967-79.
  8. Zhang, Y. et al. (2004) J Biol Chem 279, 53116-25.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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