Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

AML1 (D33G6) Rabbit mAb #4336

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC F H Endogenous 55 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

AML1 (D33G6) Rabbit mAb detects endogenous levels of total AML1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to amino acids at the amino terminus of human AML1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using AML1 (D33G6) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using AML1 (D33G6) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using AML1 (D33G6) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of Jurkat cells using AML1 (D33G6) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Background

AML1 (also known as Runx1, CBFA2 and PEBP2αB) is a member of the CBF (core binding factor) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3,4,5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21)TEL-AML and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).

  1. Wang, S. et al. (1993) Mol Cell Biol 13, 3324-3339.
  2. Ogawa, E. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 6859-6863.
  3. Okuda, T. et al. (1996) Cell 84, 321-30.
  4. Wang, Q. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 3444-3449.
  5. North, T.E. et al. (2004) Stem Cells 22, 158-168.
  6. Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-387.
  7. Tanaka, T. et al. (1996) Mol Cell Biol 16, 3967-79.
  8. Zhang, Y. et al. (2004) J Biol Chem 279, 53116-25.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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