Product Pathways - MAPK Signaling
Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4338
PhosphoSitePlus® protein, site, and accession data: MAPKAPK2
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| F | H M R Mk | Endogenous | Rabbit IgG |
Applications Key:
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4338:
- Flow
Specificity / Sensitivity
Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at Thr334.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr334 of human MAPKAPK-2. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.
Flow Cytometry
Flow cytometric analysis of THP1 cells, untreated (blue) or anisomycin-treated (green), using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (Alexa Fluor® 488 Conjugate).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 reacts with human, mouse, rat and monkey Phospho-MAPKAPK-2. CST expects that Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (Alexa Fluor® 488 conjugate) will also recognize Phospho-MAPKAPK-2 in these species.
Background
In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).
Application References
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Companion Products
- 3007 Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb
- 4320 Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 3041 Phospho-MAPKAPK-2 (Thr334) Antibody
- 3042 MAPKAPK-2 Antibody
- 2975 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
