Product Pathways - Lymphocyte Signaling
Pbx1 Antibody #4342
PhosphoSitePlus® protein, site, and accession data: PBX1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H M (R) | Endogenous | 42 kDa, 52 kDa | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Pbx1 Antibody detects endogenous levels of total Pbx1a and Pbx1b proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the amino terminus of human Pbx1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from E10.5 mouse Pbx1 heterozygote or null emrbryos. Image kindly provided by Dr. Sheldon Rowan, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School.
Western Blotting
Western blot analysis of extracts from A431, HeLa and HEL cells, using Pbx1 Antibody.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Pbx1 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).
IF-F
Immunocytochemical analysis of eye tissue from E10.5 Pbx1 heterozygous or null mouse embryos using Pbx1 Antibody (red) and Pax6 Antibody (green). Images kindly provided by Dr. Sheldon Rowan, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School.
IF-F
Immunocytochemical analysis of pancreas tissue from E10.5 Pbx1 heterozygous or null mouse embryos using Pbx1 Antibody (red) and Pdx1 Antibody (green). Blue = DAPI DNA dye. Images kindly provided by Dr. Sheldon Rowan, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School.
Background
The Pbxs (Pre-B cell leukemia transcription factors) belong to the Three Amino Acid Loop Extension (TALE) family of homeodomain containing protein (1). Pbx1 has two isoforms, Pbx1a and 1b, that are divergent in sequence at the carboxy terminus, which is a result of alternative mRNA splicing. Unlike other homeoselector genes that have restricted expression in development Pbx genes are ubiquitously expressed in both fetal and adult tissue (1). Human pre-B cell acute leukemias are frequently associated with a t(1;19)(q23;p13.3) translocation that results in a fusion between the E2A and Pbx1 genes (2,3). Pbx1 genes are not normally expressed in lymphoid tissues, and the E2A-Pbx1 fusion protein is a potent transcription factor that drives aberrant gene expression.
- Moens, C.B. and Selleri, L. (2006) Dev. Biol. 291, 193-206.
- Kamps, M.P. et al. (1990) Cell 60, 547-555.
- Lu, Q. et al. (1994) Mol. Cell Biol. 14, 3938-3948.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
