Cell Signaling Technology

Product Pathways - Translational Control

RagA (D8B5) XP™ Rabbit mAb #4357

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 30 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

RagA (D8B5) XP™ Rabbit mAb detects endogenous levels of total RagA protein. It may also detect endogenous levels of total RagB protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human RagA protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and PANC-1 cells using RagA (D8B5) XP™ Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from mIMCD3, KNRK and COS7 cells using RagA (D8B5) XP™ Rabbit mAb.

Background

mTORC1 kinase complex is a critical component in regulating cell growth (1,2). Its activity is modulated by energy levels, growth factors and amino acids (3,4). Recent studies found that RagA, RagB, RagC and RagD, the four related GTPases, interact with raptor in mTORC1 (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2).

  1. Sancak, Y. et al. (2008) Science 320, 1496-501.
  2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
  3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
  4. Wullschleger, S. et al. (2006) Cell 124, 471-84.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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