Product Pathways - Translational Control
RagA (D8B5) Rabbit mAb #4357
|W IP||H M R Mk||Endogenous||30||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
RagA (D8B5) Rabbit mAb detects endogenous levels of total RagA protein. It may also detect endogenous levels of total RagB protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human RagA protein.
Western blot analysis of extracts from 293 and PANC-1 cells using RagA (D8B5) Rabbit mAb.
The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2).
- Sancak, Y. et al. (2008) Science 320, 1496-501.
- Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
- Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
- Wullschleger, S. et al. (2006) Cell 124, 471-84.
- Sancak, Y. et al. (2008) Science 320, 1496-501. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.