Cell Signaling Technology

Product Pathways - NF-kappaB Signaling

IRAK1 Antibody (Human Specific) #4359

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H Mk Endogenous 81 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

IRAK1 (Human Specific) Antibody detects endogenous levels of total IRAK1 protein. No cross reactivity was detected with other family members at physiological conditions.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding glycine 696 of human IRAK1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, 293, and MCF7 cells using IRAK1 (Human Specific) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using IRAK1 Antibody (Human Specific).

IF-IC

IF-IC

Immunofluorescent staining of MCF7 cells, showing cytoplasmic staining, using IRAK1 (Human Specific) Antibody .


Background

Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88 and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm and activates protein kinase cascades, which include TAK1, IKKs and the stress-activated kinases (3).

  1. Dinarello, C.A. (1996) Blood 87, 2095-2147.
  2. Takaesu, G. et al. (2001) Mol. Cell. Biol. 21, 2475-2484.
  3. Janssens, S. and Beyaert, R. (2003) Mol. Cell 11, 293-302.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

Products