Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat3 (79D7) Rabbit mAb (Sepharose Bead Conjugate) #4368

Applications Reactivity Sensitivity MW (kDa) Isotype
IP H M R Mk Endogenous 79, 86 Rabbit IgG

Applications Key:  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Stat3 (79D7) Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of total Stat3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a Stat3 fusion protein corresponding to the carboxy-terminal sequence of mouse Stat3 protein.

IP

IP

Immunoprecipitation of NIH/3T3 cell lysates using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423 and Stat3 (79D7) Rabbit mAb (Sepharose Bead Conjugate). The western blot was probed using Stat3 (124H6) Mouse mAb #9139.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Stat3 (79D7) Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation assay of Stat3 proteins.

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

Application References

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Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and 7,429,487) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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