Product Pathways - NF-kB Signaling
IRAK-M Antibody #4369
|W IF-IC||H Mk||Endogenous||68||Rabbit|
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
IRAK-M antibody detects endogenous levels of total IRAK-M protein. Cross reactivity was not detected with other family members.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human IRAK-M. Antibodies were purified by protein A and peptide affinity chromagraphy.
Western blot analysis of extracts from HL-60, Raji and K562 cell lines, using IRAK-M Antibody.
Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3).
Unlike IRAK1 and IRAK4, IRAK2 and IRAK-M do not have significant kinase activity although they can still activate NF-κB when overexpressed (4). Expression of IRAK-M is more restricted compared to other family members with highest levels of expression occurring in monocytes/macrophages (4). Studies from IRAK-M knockout mice suggest that IRAK-M may play a role as a negative regulator of Toll-like receptor signaling and innate immune responses by preventing the dissociation of IRAK1 and IRAK4 from MyD88 and the subsequent formation of its complex with TRAF6 (5).
- Dinarello, C.A. (1996) Blood 87, 2095-147.
- Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84.
- Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.
- Wesche, H. et al. (1999) J. Biol. Chem. 274, 19403-10.
- Kobayashi, K. et al. (2002) Cell 110, 191-202.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 4359 IRAK1 Antibody (Human Specific)
- 4363 IRAK4 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.