Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Development

Notch1 (D6F11) XP® Rabbit mAb #4380

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC F H M R Endogenous 120, 300 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

Notch1 (D6F11) XP® Rabbit detects endogenous level of total notch1 protein. It recognizes both the full-length (~300 kDa) and the transmembrane/intracellular region NTM (~120 kDa).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln2497 of human notch1.

Western Blotting

Western Blotting

Western blot analysis of extracts from HBP-ALL, and Molt4 using Notch1 (D6F11) XP® Rabbit Ab. The full-length (FL) and transmembrane/intracellular region (NTM) are indicated.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Notch1 (D6F11) XP® Rabbit Ab (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells using Notch1 (D6F11) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4).

  1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
  2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
  3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
  4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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