Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

MTSS1 (P549) Antibody #4385

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 95-105 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MTSS1 (P549) Antibody detects endogenous levels of total MTSS1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Pro549 of human MTSS1. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MTSS1 (P549) Antibody.

Background

Metastasis suppressor 1 (MTSS1) is a multi-functional scaffold protein that was initially discovered using a differential display technique that uncovered proteins missing from bladder cancer cell lines (1,2). MTSS1 (also known as Missing in Metastasis or MIM) is a cytoskeletal remodeling protein that contains a C-terminal WH2 actin-binding motif (1,3). Presence of an IMD (IRSp53/MIM homology) domain allows MTSS1 to induce F-actin bundling and filopodia formation in cells (4). MTSS1 binds to and activates Rac, a protein known to promote the formation of filopodia and lamellipodia (5). The receptor tyrosine phosphatase δ (PTPRD) is associated with MTSS1 and is required for MTSS1-dependent cytoskeletal change (6,7). MTSS1 is a Shh responsive gene that can help regulate Gli transcriptional activity (8). Transcription of MTSS1 is regulated by DNA methylation (9).

  1. Lee, Y.G. et al. Neoplasia 4, 291-4.
  2. Machesky, L.M. and Johnston, S.A. (2007) J Mol Med 85, 569-76.
  3. Mattila, P.K. et al. (2003) J Biol Chem 278, 8452-9.
  4. Yamagishi, A. et al. (2004) J Biol Chem 279, 14929-36.
  5. Bompard, G. et al. (2005) J Cell Sci 118, 5393-403.
  6. Woodings, J.A. et al. (2003) Biochem J 371, 463-71.
  7. Gonzalez-Quevedo, R. et al. (2005) J Cell Biol 168, 453-63.
  8. Callahan, C.A. et al. (2004) Genes Dev 18, 2724-9.
  9. Utikal, J. et al. (2006) Int J Cancer 119, 2287-93.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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