Cell Signaling Technology

Product Pathways - Translational Control

ATP2A2/SERCA2 Antibody #4388

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 114 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ATP2A2/SERCA2 Antibody detects endogenous levels of total ATP2A2/SERCA2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence around Leu32 of human ATP2A2/SERCA2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using ATP2A2/SERCA2 Antibody.

Background

The ATP2A2 (SERCA2) calcium pump is one of several sarcoplasmic and endoplasmic reticulum Ca2+-ATPases responsible for regulating calcium transport across intracellular membranes (1). Multiple isoforms have been isolated, with ATP2A2a (SERCA2a) found predominantly in the sarcoplasmic reticulum of muscle cells and ATP2A2b (SERCA2b) more ubiquitously expressed in the endoplasmic reticulum of most cell types (2). An isoform containing a truncated carboxy region (ATP2A2c) is expressed in epithelial and hematopoietic cell lines and may be involved in monocyte differentiation (3). Post-translational modification of ATP2A2 (SERCA2), including phosphorylation and tyrosine nitration, modify Ca2+ -ATPase activity and calcium transport (4,5). Mutation in the corresponding ATP2A2 (SERCA2) gene results in Darier disease, a skin disorder characterized by the presence of dark, keratotic papules or rash found on the head and torso (6).

  1. Vangheluwe, P. et al. (2005) Cell Calcium 38, 291-302.
  2. de Smedt, H. et al. (1991) J Biol Chem 266, 7092-5.
  3. Gélébart, P. et al. (2003) Biochem Biophys Res Commun 303, 676-84.
  4. Hawkins, C. et al. (1995) Mol Cell Biochem 142, 131-8.
  5. Viner, R.I. et al. (1999) Biochem J 340 ( Pt 3), 657-69.
  6. Sakuntabhai, A. et al. (1999) Hum Mol Genet 8, 1611-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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