Product Pathways - Motif Antibodies
Phospho-MAPK Substrates (PXTP) (46G11) Rabbit mAb #4391
| Applications | Reactivity | Source | Isotype |
|---|---|---|---|
| W IP IHC-P F E-P | All | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
F=Flow Cytometry
E-P=ELISA (Peptide)
Reactivity Key: All=All species expected
Specificity / Sensitivity
Phospho-MAPK Substrates (PXT*P) (46G11) Rabbit mAb detects phospho-threonine in a PXT*P motif. The antibody is phospho-specific, and does not react with phospho-serine- or phospho-tyrosine-containing peptides/proteins. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with synthetic phospho-MAPK/CDK substrate peptides (KLH-coupled).
Western Blotting
Western blot analysis of extracts from serum-starved A431 cells, phosphorylated in vitro with MAPK or treated in culture with EGF, using Phospho-MAPK Substrate (PXTP) (46G11) Rabbit mAb. Lysis buffer: 1.0% Triton X-100 (lanes 1 and 2), 2.0% SDS (lanes 3 and 4).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-MAPK Substrates (PXTP) (46G11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-MAPK Substrates (PXTP) (46G11) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or TPA-treated (green), using Phospho-MAPK Substrates (PXTP) (46G11) Rabbit mAb compared to a nonspecific negative control antibody (red). Parallel samples were tested without (A) or with (B) lambda phosphatase treatment prior to staining.
ELISA-Peptide
Phospho-MAPK Substrate (PXTP) (46G11) Rabbit mAb DELFIA® Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. T* denotes phosphorylated threonine. (DELFIA® is a registered trademark of PerkinElmer, Inc.)
Background
The MAPK and CDK families of serine/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-3). MAPK phosphorylates substrates with the concensus sequence PX(S/T)P, and CDKs phosphorylate substrates containing the concensus sequence (S/T)PXK/R. CST has developed antibodies that bind to phospho-threonine followed by proline, motifs PXS*/T*P and/or S*PXK/R, for use in the study and discovery of new MAPK and CDK substrates (4,5).
- Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
- Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.
- Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
- Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.
- Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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Rabbit Monoclonals Produced Using Technology from Epitomics, Inc. Under U.S. patent No. 5,675,063.License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commerical licensing terms please contact CST Business Development at cbunker@cellsignal.com.
