Cell Signaling Technology

Product Pathways - Neuroscience

STEP (23E5) Mouse mAb #4396

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-F M R Endogenous 46, 61 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

STEP (23E5) Mouse mAb detects endogenous levels of total STEP46 and STEP61 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of rat STEP46 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from adult rat and fetal rat brain using STEP (23E5) Mouse mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of rat hippocampus (left) and striatum (right) using STEP (23E5) Mouse mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (blue). Red = propidium iodide (fluorescent DNA dye).

Background

Striatal enriched phosphatase (STEP, also known as PTPN5), is a protein tyrosine phosphatase expressed in dopaminoceptive neurons of the central nervous system (1). Alternative splicing produces the cytosolic STEP46 and the membrane-associated STEP61 isoforms of STEP. Dopamine activates D1 receptors and PKA, which in turn phosphorylate both isoforms of STEP. Phosphorylation of STEP61 occurs at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49 (equivalent to Ser221 of STEP61) (2). NMDA-mediated activation of STEP is an important mechanism for regulation of Erk activity in neurons (3). Furthermore, STEP is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). Due to its importance in cognitive function, STEP may play a role in Alzheimer's disease (1).

  1. Braithwaite, S.P. et al. (2006) Trends Neurosci 29, 452-8.
  2. Paul, S. et al. (2000) J Neurosci 20, 5630-8.
  3. Paul, S. et al. (2003) Nat Neurosci 6, 34-42.
  4. Braithwaite, S.P. et al. (2006) Eur J Neurosci 23, 2847-56.
  5. Zhang, Y. et al. (2008) J Neurosci 28, 10561-6.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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