Product Pathways - Neuroscience
STEP (23E5) Mouse mAb #4396
|4396S||100 µl (10 western blots)||---||In Stock||---|
|4396||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Mouse, Rat||Endogenous||46, 61||Mouse IgG1|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen)
Specificity / Sensitivity
STEP (23E5) Mouse mAb detects endogenous levels of total STEP46 and STEP61 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of rat STEP46 protein.
Western blot analysis of extracts from adult rat and fetal rat brain using STEP (23E5) Mouse mAb.
Striatal enriched phosphatase (STEP, also known as PTPN5), is a protein tyrosine phosphatase expressed in dopaminoceptive neurons of the central nervous system (1). Alternative splicing produces the cytosolic STEP46 and the membrane-associated STEP61 isoforms of STEP. Dopamine activates D1 receptors and PKA, which in turn phosphorylate both isoforms of STEP. Phosphorylation of STEP61 occurs at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49 (equivalent to Ser221 of STEP61) (2). NMDA-mediated activation of STEP is an important mechanism for regulation of Erk activity in neurons (3). Furthermore, STEP is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). Due to its importance in cognitive function, STEP may play a role in Alzheimer's disease (1).
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