Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

ACVR1 Antibody #4398

Applications Reactivity Sensitivity MW (kDa) Source
W H M Mk Endogenous 57 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ACVR1 Antibody detects endogenous levels of total ACVR1 protein. The antibody also cross-reacts with 35 kDa and 95 kDa bands of unknown origin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ACVR1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ACVR1 Antibody.

Background

The activin A receptor type 1 (ACVR1) is a membrane associated serine/threonine kinase receptor that binds activin A and related TGF-β superfamily growth factors, including bone morphogenetic protein 2 (BMP2) and BMP4, 6 and 7 (1-3). Functional activin receptors are composed of a pair of type I and type II activin receptor proteins. Following ligand binding, ACVR1 is phosphorylated by its type II binding partner, which allows recruitment and activation of receptor regulated SMAD (R-Smad) proteins (4). Phosphorylation of R-Smad proteins results in their translocation to the nucleus where R-Smads stimulate transcription of target genes (5). Mutations in the corresponding ACVR1 gene are associated with the genetic developmental disorders hereditary hemorrhagic telangiectasia and fibrodysplasia ossificans progressive (6,7).

  1. Attisano, L. et al. (1993) Cell 75, 671-80.
  2. Macías-Silva, M. et al. (1998) J Biol Chem 273, 25628-36.
  3. Ho, C.C. and Bernard, D.J. (2009) Biol Reprod 81, 133-41.
  4. Attisano, L. et al. (1996) Mol Cell Biol 16, 1066-73.
  5. Attisano, L. and Wrana, J.L. (2000) Curr Opin Cell Biol 12, 235-43.
  6. Shore, E.M. et al. (2006) Nat Genet 38, 525-7.
  7. Letteboer, T.G. et al. (2005) Hum Genet 116, 8-16.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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