Cell Signaling Technology

Product Pathways - Protein Stability

MIB1 Antibody #4400

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 110 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MIB1 Antibody detects endogenous levels of total MIB1 protein. This antibody does not recognize the Ki-67 (MK167) protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human MIB1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T and K-562 cells using MIB1 Antibody.

Background

Mindbomb homolog 1 (MIB1) is an E3 ligase that facilitates the ubiquitination and the subsequent endocytosis of the Notch ligands, Delta and Jagged (1,2). MIB1 appears to promote the ubiquitination and degradation of death-associated protein kinase (DAPK1) in vitro (3). Expression of MIB1 is seen in both adult and embryonic murine tissues (4). Recently, MIB1 was reported to regulate the extrinsic cell death pathway by binding to cellular FLICE-like inhibitory proteins (cFLIP-L and cFLIP-S), which reduces the interaction of caspase-8 with cFLIP and leads to cell death (5). MIB1 is also involved in T and marginal zone B (MZB) cell development in the lymphopoietic niches (6).

  1. Koo, B.K. et al. (2005) Development 132, 3459-70.
  2. Matsuda, M. and Chitnis, A.B. (2009) Development 136, 197-206.
  3. Zhang, L. et al. (2007) J Biol Chem 282, 11795-804.
  4. Jin, Y. et al. (2002) J Biol Chem 277, 46980-6.
  5. Zhang, L. and Gallagher, P.J. (2009) Am J Physiol Cell Physiol 297, C1275-83.
  6. Song, R. et al. (2008) J Exp Med 205, 2525-36.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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