Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Development

FoxP1 (D35D10) XP® Rabbit mAb #4402

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P F H M R Mk Endogenous 82-90 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FoxP1 (D35D10) XP® Rabbit mAb detects endogenous levels of total FoxP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu584 of human FoxP1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FoxP1 (D35D10) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FoxP1 (D35D10) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MOLT-4 cells using FoxP1 (D35D10) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


Background

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response, and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5).

FoxP1 plays an important role in cardiac valve and B cell development. Targeted deletion of FoxP1 in mice results in embryonic death at E14.5 with severe defects in cardiac outflow development, endocardial cushion morphogenesis, and myocyte proliferation and maturation (6). The FoxP1 gene is located on chromosome 3p14.1 and loss of heterozygosity of this region in multiple cancer types suggests a role for FoxP1 as a tumor suppressor (7). Alternatively, FoxP1 may also act as an oncogene as it is highly expressed in a variety of B cell malignancies and is frequently involved in chromosome translocation (8,9).

  1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
  2. Shu, W. et al. (2001) J Biol Chem 276, 27488-97.
  3. Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8.
  4. Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65.
  5. Li, S. et al. (2004) Mol Cell Biol 24, 809-22.
  6. Wang, B. et al. (2004) Development 131, 4477-87.
  7. Banham, A.H. et al. (2001) Cancer Res 61, 8820-9.
  8. Wlodarska, I. et al. (2005) Leukemia 19, 1299-305.
  9. Goatly, A. et al. (2008) Mod Pathol 21, 902-11.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products