Product Pathways - NF-kappaB Signaling

TWEAK Receptor/Fn14 Antibody #4403

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H M R B	Endogenous	14-17	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

TWEAK Receptor/Fn14 Antibody detects endogenous levels of total TWEAK Receptor/Fn14 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxy terminus of the TWEAK Receptor/Fn14. Antibodies were purified by protein A and peptide affinity chromatography.

Background

The receptor for TWEAK, fibroblast growth factor-inducible 14 (Fn14), is a member of the TNF superfamily of receptors thought to be involved in cell growth, adhesion and migration (1). Its expression is regulated by a number of different growth factors (1,2). Elevated expression is also seen in some cancers including hepatocellular carcinomas (3), glioblastomas (4), and pancreatic cancer (5). The receptor contains a binding site for members of the TNFR-associated factor (TRAF) family that promotes the activation of NF-κB (1,6). Recent studies have suggeted some therapeutic potential of TWEAK and its receptor signaling in regards to autoimmunity and cancer treatment (7-9).

1. Wiley, S.R. et al. (2001) Immunity 15, 837-46.
2. Nakayama, M. et al. (2003) J. Immunol. 170, 341-8.
3. Feng, S.L. et al. (2000) Am. J. Pathol. 156, 1253-61.
4. Tran, N.L. et al. (2003) Am. J. Pathol. 162, 1313-21.
5. Han, H. et al. (2002) Cancer Res. 62, 2890-6.
6. Brown, S.A. et al. (2003) Biochem J. 371, 395-403.
7. Desplat-Jego, S. et al. (2005) Clin. Immunol. 117, 15-23.
8. Mueller, A.M. et al. (2005) J. Neuroimmunol. 159, 55-65.
9. Winkles, J.A. et al. (2005) Cancer Lett., May 7, [Epub ahead of print].

Application References

• Dogra, C. et al. (2007) J Biol Chem 282, 15000-10. This article references the use of TWEAK Receptor/Fn14 Antibody in the following applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.