Cell Signaling Technology

Product Pathways - NF-kB Signaling

TWEAK Receptor/Fn14 Antibody #4403

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R B Endogenous 14-17 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TWEAK Receptor/Fn14 Antibody detects endogenous levels of total TWEAK Receptor/Fn14 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of the TWEAK Receptor/Fn14. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-1080, PC3 and HT29 cell lines, using TWEAK Receptor/Fn14 Antibody.

Background

The receptor for TWEAK, fibroblast growth factor-inducible 14 (Fn14), is a member of the TNF superfamily of receptors thought to be involved in cell growth, adhesion and migration (1). Its expression is regulated by a number of different growth factors (1,2). Elevated expression is also seen in some cancers including hepatocellular carcinomas (3), glioblastomas (4), and pancreatic cancer (5). The receptor contains a binding site for members of the TNFR-associated factor (TRAF) family that promotes the activation of NF-κB (1,6). Recent studies have suggeted some therapeutic potential of TWEAK and its receptor signaling in regards to autoimmunity and cancer treatment (7-9).

  1. Wiley, S.R. et al. (2001) Immunity 15, 837-46.
  2. Nakayama, M. et al. (2003) J. Immunol. 170, 341-8.
  3. Feng, S.L. et al. (2000) Am. J. Pathol. 156, 1253-61.
  4. Tran, N.L. et al. (2003) Am. J. Pathol. 162, 1313-21.
  5. Han, H. et al. (2002) Cancer Res. 62, 2890-6.
  6. Brown, S.A. et al. (2003) Biochem J. 371, 395-403.
  7. Desplat-Jégo, S. et al. (2005) Clin. Immunol. 117, 15-23.
  8. Mueller, A.M. et al. (2005) J. Neuroimmunol. 159, 55-65.
  9. Winkles, J.A. et al. (2006) Cancer Lett 235, 11-7.

Application References

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Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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