Cell Signaling Technology

Product Pathways - NF-kappaB Signaling

TWEAK Receptor/Fn14 Antibody #4403

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R B Endogenous 14-17 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

TWEAK Receptor/Fn14 Antibody detects endogenous levels of total TWEAK Receptor/Fn14 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxy terminus of the TWEAK Receptor/Fn14. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-1080, PC3 and HT29 cell lines, using TWEAK Receptor/Fn14 Antibody.

Background

The receptor for TWEAK, fibroblast growth factor-inducible 14 (Fn14), is a member of the TNF superfamily of receptors thought to be involved in cell growth, adhesion and migration (1). Its expression is regulated by a number of different growth factors (1,2). Elevated expression is also seen in some cancers including hepatocellular carcinomas (3), glioblastomas (4), and pancreatic cancer (5). The receptor contains a binding site for members of the TNFR-associated factor (TRAF) family that promotes the activation of NF-κB (1,6). Recent studies have suggeted some therapeutic potential of TWEAK and its receptor signaling in regards to autoimmunity and cancer treatment (7-9).

  1. Wiley, S.R. et al. (2001) Immunity 15, 837-46.
  2. Nakayama, M. et al. (2003) J. Immunol. 170, 341-8.
  3. Feng, S.L. et al. (2000) Am. J. Pathol. 156, 1253-61.
  4. Tran, N.L. et al. (2003) Am. J. Pathol. 162, 1313-21.
  5. Han, H. et al. (2002) Cancer Res. 62, 2890-6.
  6. Brown, S.A. et al. (2003) Biochem J. 371, 395-403.
  7. Desplat-Jego, S. et al. (2005) Clin. Immunol. 117, 15-23.
  8. Mueller, A.M. et al. (2005) J. Neuroimmunol. 159, 55-65.
  9. Winkles, J.A. et al. (2005) Cancer Lett., May 7, [Epub ahead of print].

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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