Product Pathways - NF-kB Signaling

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TWEAK Receptor/Fn14 Antibody #4403

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H M R B	Endogenous	14-17	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

4403:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

TWEAK Receptor/Fn14 Antibody detects endogenous levels of total TWEAK Receptor/Fn14 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of the TWEAK Receptor/Fn14. Antibodies were purified by protein A and peptide affinity chromatography.

Background

The receptor for TWEAK, fibroblast growth factor-inducible 14 (Fn14), is a member of the TNF superfamily of receptors thought to be involved in cell growth, adhesion and migration (1). Its expression is regulated by a number of different growth factors (1,2). Elevated expression is also seen in some cancers including hepatocellular carcinomas (3), glioblastomas (4), and pancreatic cancer (5). The receptor contains a binding site for members of the TNFR-associated factor (TRAF) family that promotes the activation of NF-κB (1,6). Recent studies have suggeted some therapeutic potential of TWEAK and its receptor signaling in regards to autoimmunity and cancer treatment (7-9).

1. Wiley, S.R. et al. (2001) Immunity 15, 837-46.
2. Nakayama, M. et al. (2003) J. Immunol. 170, 341-8.
3. Feng, S.L. et al. (2000) Am. J. Pathol. 156, 1253-61.
4. Tran, N.L. et al. (2003) Am. J. Pathol. 162, 1313-21.
5. Han, H. et al. (2002) Cancer Res. 62, 2890-6.
6. Brown, S.A. et al. (2003) Biochem J. 371, 395-403.
7. Desplat-Jégo, S. et al. (2005) Clin. Immunol. 117, 15-23.
8. Mueller, A.M. et al. (2005) J. Neuroimmunol. 159, 55-65.
9. Winkles, J.A. et al. (2006) Cancer Lett 235, 11-7.

Application References

• Dogra, C. et al. (2007) J Biol Chem 282, 15000-10. Applications: Western Blotting
• Shan, W. et al. (2008) Toxicol Sci 105, 418-28. Applications: Western Blotting
• Vince, J.E. et al. (2008) J Cell Biol 182, 171-84. Applications: Western Blotting
• Ikner, A. and Ashkenazi, A. (2011) J Biol Chem 286, 21546-54. Applications: IP Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 3707 TNF-α Antibody
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7074 Anti-rabbit IgG, HRP-linked Antibody

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For Research Use Only. Not For Use In Diagnostic Procedures.