Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-EGF Receptor (Tyr1148) Antibody #4404
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P | H Mk | Endogenous | 175 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-EGF Receptor (Tyr1148) Antibody detects endogenous levels of EGF receptor only when phosphorylated at tyrosine 1148. This antibody does not cross-react with other tyrosine-phosphorylated ErbB family members.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr1148 of human EGF receptor. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from A431 cells, untreated or EGF-stimulated, using Phospho-EGF Receptor (Tyr1148) Antibody (upper) or EGF Receptor Antibody #2232 (lower).
Western Blotting
Western blot analysis of various cell lysates containing activated EGFR, ErbB2, ErbB3 and ErbB4 proteins, using Phospho-EGF Receptor (Tyr1148) Antibody (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower).
IHC-P (paraffin)
Immunohistochemical staining of phosphorylated EGF Receptor in paraffin-embedded human breast carcinoma, showing membrane localization using Phospho-EGF Receptor (Tyr1148) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 cells, untreated (left), EGF-treated (middle) or EGF and Tarceva ®- treated (right), using Phospho-EGF Receptor (Tyr1148) Antibody.
Background
The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated residues (Tyr1148 and Tyr1173) provides a docking site for the SHC scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutations to either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).
- Hackel, P.O. et al. (1999) Curr. Opin. Cell Biol. 11, 184-189.
- Zwick, E. et al. (1999) Trends Pharmacol. Sci. 20, 408-412.
- Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-1054.
- Hubbard, S.R. et al. (1994) Nature 372, 746-754.
- Biscardi, J.S. et al. (1999) J. Biol. Chem. 274, 8335-8343.
- Emlet, D.R. et al. (1997) J. Biol. Chem. 272, 4079-4086.
- Levkowitz, G. et al. (1999) Mol. Cell 4, 1029-1040.
- Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-1866.
- Rojas, M. et al. (1996) J. Biol. Chem. 271, 27456-27461.
- Feinmesser, R.L. et al. (1999) J. Biol. Chem. 274, 16168-16173.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2232 EGF Receptor Antibody
- 2234 Phospho-EGF Receptor (Tyr1068) Antibody
- 2235 Phospho-EGF Receptor (Tyr992) Antibody
- 2236 Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb
- 2237 Phospho-EGF Receptor (Tyr1045) Antibody
- 9922 Phospho-EGF Receptor Antibody Sampler Kit
- 8102 SignalSlide™ Phospho-EGF Receptor (Tyr1173) IHC Controls
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
