Secondary Antibodies
Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) #4409
Specificity / Sensitivity
F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using S6 Ribosomal Protein (54D2) Mouse mAb #2317 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
High Content Screening
High content analysis of C2C12 cells exposed to varying concentrations of LY294002 #9901 for 2 hours. With increasing concentrations of LY294002, a significant decrease (~20 fold) in phospho-S6 Ribosomal protein (Ser235/236) signal as compared to the untreated control was observed. When using phospho-S6 Ribosomal protein as a measurement, the IC50 of this compound was 2.3 μM. Data was generated on the Acumen® HCS platform using Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate).
Description
Anti-Mouse IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.
Directions for Use
The optimal dilution of the anti-species antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescent applications.
Applications
This product has been optimized for use as a secondary antibody in immunofluorescent applications.
Background
Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4753 Cellular Localization IF Antibody Sampler Kit
- 4084 DRAQ5®
- 4408 Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
- 4410 Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
- 4412 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
- 4413 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
- 4414 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
- 4416 Anti-rat IgG (H+L), (Alexa Fluor® 488 Conjugate)
- 4417 Anti-rat IgG (H+L), (Alexa Fluor® 555 Conjugate)
- 4418 Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate)
- 5425 Normal Goat Serum
This product is provided under an agreement between Life Technologies Corporation and Cell Signaling Technology, Inc., and the manufacture, use, sale or import of antibody conjugate in this product is subject to one or more US patents and corresponding non-US equivalents, owned or controlled by Life Technologies Corporation or its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cellular Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
