Cell Signaling Technology

Product Pathways - Translational Control

Paip2A Antibody #4422

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 25 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Paip2A Antibody detects endogenous levels of total Paip2A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human Paip2A. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse testis and human testis using Paip2A Antibody.

Background

The eukaryotic mRNA 3' poly(A) tail interacts with the 5' cap structure to regulate translation of mRNA transcripts (1). The eIF4G translation initiation factor associates with poly(A) binding protein (PABP) to circularize mRNA (1,2). Paip1 is a PABP interacting protein that binds eIF4A to stimulate translation (3). A second PABP binding protein, Paip2, inhibits translation by interfering with PABP function. Specifically, Paip2 reduces the affinity of PABP for poly(A) RNA and competes with Paip1 for PABP binding (1,2).

  1. Khaleghpour, K. et al. (2001) Mol Cell 7, 205-16.
  2. Yoshida, M. et al. (2006) EMBO J 25, 1934-44.
  3. Craig, A.W. et al. (1998) Nature 392, 520-3.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products