Product Pathways - NF-kappaB Signaling
CARD11 (1D12) Rabbit mAb #4435
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W | H M (R) (Mk) | Endogenous | 130 | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
CARD11 (1D12) Rabbit mAb detects endogenous levels of total CARD11 protein.
Source / Purification
Rabbit monoclonal antibodies are prepared from rabbits immunized with a synthetic peptide (KLH-coupled) corresponding to residues surrounding threonine 175 of CARD11.
Western Blotting
Western blot analysis of extracts from K562, SR and NK-92 cell lines, using CARD11 (1D12) Rabbit mAb.
Background
CARD11/Carma1/Bimp3 belongs to the MAGUK (membrane-associated guanylate kinase) family which typically function as molecular scaffolds in the assembly of multiprotein complexes (1,2). MAGUK family members contain an SH3 domain, a PDZ domain and a GuK domain homologous to guanylate kinase. In addition, CARD11 contains an amino-terminal CARD domain (caspase recruitment domain). This domain plays an important role in forming interactions with a number of proteins containing CARD domains that are involved in regulating apoptosis and NF-κB activation. CARD11 is predominately expressed in lymphocytes (1,2) and associates with the CARD domain of Bcl10. When overexpressed, CARD11 leads to the phosphorylation of Bcl10 and activation of NF-κB (1,2). CARD11 is constitutively associated with lipid rafts and is thought to function by recruiting Bcl10 and MALT1 and triggering the phosphorylation of IKKs (3,4). Several studies using the genetic disruption of CARD11 or dominant-negative mutations have demonstrated that it plays a critical role in NF-κB activation and lymphocyte signaling (4-7).
- Bertin, J. et al. (2001) J. Biol. Chem. 276, 11877?11882.
- Gaide, O. et al. (2001) FEBS Lett. 496, 121-127.
- Stilo, R. et al. (2004) J. Biol. Chem. 279, 34323-34331.
- Wang, D. et al. (2002) Nat. Immunol. 3, 830-835.
- Jun, J.E. et al. (2003) Immunity 18, 751-762.
- Hara, H. et al. (2003) Immunity 18, 763-775.
- Gaide, O. et al. (2002) Nat. Immunol. 3, 836-843.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
