Cell Signaling Technology

Product Pathways - NF-kB Signaling

CARD11 (1D12) Rabbit mAb #4435

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M (R) (Mk) Endogenous 130 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CARD11 (1D12) Rabbit mAb detects endogenous levels of total CARD11 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding threonine 175 of CARD11.

Western Blotting

Western Blotting

Western blot analysis of extracts from K562, SR and NK-92 cell lines, using CARD11 (1D12) Rabbit mAb.

Background

CARD11/Carma1/Bimp3 belongs to the MAGUK (membrane-associated guanylate kinase) family that typically function as molecular scaffolds in the assembly of multiprotein complexes (1,2). MAGUK family members contain an SH3 domain, a PDZ domain and a GuK domain homologous to guanylate kinase. In addition, CARD11 contains an amino-terminal CARD domain (caspase recruitment domain). This domain plays an important role in forming interactions with a number of proteins containing CARD domains that are involved in regulating apoptosis and NF-κB activation. CARD11 is predominately expressed in lymphocytes (1,2) and associates with the CARD domain of Bcl10. When overexpressed, CARD11 leads to the phosphorylation of Bcl10 and activation of NF-κB (1,2). CARD11 is constitutively associated with lipid rafts and is thought to function by recruiting Bcl10 and MALT1 and triggering the phosphorylation of IKKs (3,4). Several studies using the genetic disruption of CARD11 or dominant-negative mutations have demonstrated that it plays a critical role in NF-κB activation and lymphocyte signaling (4-7).

  1. Bertin, J. et al. (2001) J. Biol. Chem. 276, 11877–11882.
  2. Gaide, O. et al. (2001) FEBS Lett. 496, 121-127.
  3. Stilo, R. et al. (2004) J. Biol. Chem. 279, 34323-34331.
  4. Wang, D. et al. (2002) Nat. Immunol. 3, 830-835.
  5. Jun, J.E. et al. (2003) Immunity 18, 751-762.
  6. Hara, H. et al. (2003) Immunity 18, 763-775.
  7. Gaide, O. et al. (2002) Nat. Immunol. 3, 836-843.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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