Product Pathways - NF-kappaB Signaling

CARD11 Antibody #4440

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W	H M R	Endogenous	130	Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

CARD11 Antibody detects endogenous levels of total CARD11 protein. Cross-reactivity was not detected with other family members at physiological conditions.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding methionine 362 of human CARD11. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CARD11/Carma1/Bimp3 belongs to the MAGUK (membrane-associated guanylate kinase) family which typically function as molecular scaffolds in the assembly of multiprotein complexes (1,2). MAGUK family members contain an SH3 domain, a PDZ domain and a GuK domain homologous to guanylate kinase. In addition, CARD11 contains an amino-terminal CARD domain (caspase recruitment domain). This domain plays an important role in forming interactions with a number of proteins containing CARD domains that are involved in regulating apoptosis and NF-κB activation. CARD11 is predominately expressed in lymphocytes (1,2) and associates with the CARD domain of Bcl10. When overexpressed, CARD11 leads to the phosphorylation of Bcl10 and activation of NF-κB (1,2). CARD11 is constitutively associated with lipid rafts and is thought to function by recruiting Bcl10 and MALT1 and triggering the phosphorylation of IKKs (3,4). Several studies using the genetic disruption of CARD11 or dominant-negative mutations have demonstrated that it plays a critical role in NF-κB activation and lymphocyte signaling (4-7).

1. Bertin, J. et al. (2001) J. Biol. Chem. 276, 11877?11882.
2. Gaide, O. et al. (2001) FEBS Lett. 496, 121-127.
3. Stilo, R. et al. (2004) J. Biol. Chem. 279, 34323-34331.
4. Wang, D. et al. (2002) Nat. Immunol. 3, 830-835.
5. Jun, J.E. et al. (2003) Immunity 18, 751-762.
6. Hara, H. et al. (2003) Immunity 18, 763-775.
7. Gaide, O. et al. (2002) Nat. Immunol. 3, 836-843.

Application References

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.