Product Pathways - Jak/Stat Pathway
Phospho-Stat2 (Tyr690) Antibody #4441
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H (R) (B) | Endogenous | 113 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
R=Rat
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Stat2 Antibody detects endogenous levels of human Stat2 only when phosphorylated at Tyr690. The antibody does not significantly cross-react with corresponding phospho-tyrosine sites on other Stat proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr690 of human Stat2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or IFN-a-treated (100 ng/ml,15 minutes), using Stat2 Antibody (upper) or Phospho-Stat2 (Tyr690) Antibody (lower).
Background
Stat2 (113-kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via assocations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transciption from the ISRE (5).
- Fu, X. et al. (1992) Biochemistry 89, 7840-7843.
- Ihle, J.N. (2001) Curr.Opin.Cell Biol. 13, 211-217.
- Park, C. et al. (2000) Immunity 13, 795-804.
- Improta, T. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4776-4780.
- Horvath, C. et al. (1996) Molecular and Cellular Biology 16, 6957-6964.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4594 Stat2 Antibody
- 4597 Stat2 Antibody (Mouse Specific)
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
