Cell Signaling Technology

Product Pathways - Development

AFP (D12C1) Rabbit mAb #4448

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H (Mk) Endogenous 65 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

AFP (D12C1) Rabbit mAb recognizes endogenous levels of total AFP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AFP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Hep G2 cells using AFP (D12C1) Rabbit mAb.

Background

Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma (3).

  1. Meier, V. et al. (2006) Comp Hepatol 5, 2.
  2. Warnes, T.W. and Smith, A. (1987) Baillieres Clin Gastroenterol 1, 63-89.
  3. Peng, S.Y. et al. (2004) Int J Cancer 112, 44-50.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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