Product Pathways - Chromatin Regulation / Epigenetics
RAD21 (L611) Antibody #4449
|4449S||100 µl (10 western blots)||---||In Stock||---|
|4449||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||130||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Bovine, Horse.
Specificity / Sensitivity
RAD21 (L611) Antibody detects endogenous levels of total RAD21 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human RAD21 protein. Antibodies are purified by protein A and peptide affinity chromatography.
The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64 kDa carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2).
- Peters, J.M. et al. (2008) Genes Dev 22, 3089-114.
- Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35.
- Hoque, M.T. and Ishikawa, F. (2001) J Biol Chem 276, 5059-67.
- Hauf, S. et al. (2005) PLoS Biol 3, e69.
- Hauf, S. et al. (2001) Science 293, 1320-3.
- Pati, D. et al. (2002) Mol Cell Biol 22, 8267-77.
- Chen, F. et al. (2002) J Biol Chem 277, 16775-81.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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