Cell Signaling Technology

Product Pathways - Metabolism

CCTα Antibody #4454

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 42 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CCTα Antibody detects endogenous levels of total CCTα protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CCTα protein. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-1080 and K-562 cells using CCTα Antibody.

Background

CTP:phosphocholine cytidylyltransferase (CCT) is a critical enzyme that regulates the CDP-choline pathway for the biosynthesis of phosphatidylcholine. Three distinct CCT isoforms are found in mammals, including CCTα, CCTβ2, and CCTβ3 (1,2). CCTα is the major isoform that is expressed in most tissues (3). CCTα is essential in the synthesis and secretion of surfactant by alveolar epithelial cells and is important in maintaining the phosphatidylcholine level that regulates lipoprotein assembly and secretion in hepatocytes (4,5). CCTα is a major component in membrane biogenesis during cytokine secretion by stimulated macrophages (6). Monoubiquitination of CCTα prevents it from entering the nucleus and leads to its degradation by lysosome (7).

  1. Jackowski, S. and Fagone, P. (2005) J Biol Chem 280, 853-6.
  2. Clement, J.M. and Kent, C. (1999) Biochem Biophys Res Commun 257, 643-50.
  3. Karim, M. et al. (2003) Biochim Biophys Acta 1633, 1-12.
  4. Tian, Y. et al. (2007) Mol Cell Biol 27, 975-82.
  5. Jacobs, R.L. et al. (2004) J Biol Chem 279, 47402-10.
  6. Tian, Y. et al. (2008) J Cell Biol 181, 945-57.
  7. Chen, B.B. and Mallampalli, R.K. (2009) Mol Cell Biol 29, 3062-75.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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