Cell Signaling Technology

Product Pathways - Neuroscience

Nna1 Antibody #4456

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 145 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Nna1 Antibody detects endogenous levels of total Nna1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp1175 of human Nna1 protein. Antibodies are purified by protein A and peptide affinity chromatography

Western Blotting

Western Blotting

Western blot analysis of extracts from fetal and adult rat brain using Nna1 Antibody.

Background

Nervous system nuclear protein induced by axotomy 1 (Nna1, AGTPBP1 or cytosolic carboxypeptidase-1) is related to zinc carboxypeptidases and contains an ATP/GTP binding motif, a basic and a bipartite nuclear localization signal. Nna1 expression is rapidly induced in motor neurons following axotomy and down-regulated following reinnervation, which is consistent with abundant Nna1 expression in differentiating neurons and the absence of Nna1 in CNS proliferative zones (1). Furthermore, Purkinje cell degeneration is characterized by adult onset neurodegeneration resulting from Nna1 gene mutations, implicating Nna1 in mechanisms common to degeneration and regeneration (2).

  1. Harris, A. et al. (2000) Mol Cell Neurosci 16, 578-96.
  2. Fernandez-Gonzalez, A. et al. (2002) Science 295, 1904-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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