Cell Signaling Technology

Product Pathways - Metabolism

ARK5 Antibody #4458

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk (B) Endogenous 78 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ARK5 Antibody detects endogenous levels of total ARK5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ARK5. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from L929 and C33A cells, using ARK5 Antibody.

Background

AMP-activated protein kinases (AMPKs) constitute a serine/threonine protein kinase family, which is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPKα1, AMPKα2, MELK and SNARK are the catalytic subunits in the family (2). Recently, AMPK-related protein kinase ARK5 was identified, which shares 84% similarity with the sequence of the catalytic domain of SNARK (2). In vitro, Akt phosphorylates ARK5 at Ser600. This phosphorylation activates ARK5, which in turn results in the increased phosphorylation of the SAMS peptide, an AMPK consensus substrate (2). In vivo experiments showed that Akt-activated ARK5 is critical for the survival of cells under glucose starvation (2). Furthermore, studies also linked ARK5 to tumor invasion (3, 4). Overexpressed ARK5 leads to higher tumor growth rate (3). For example, the overexpression of ARK5 in pancreatic tumor cell line PANC-1 significantly elevates its metastasis in the liver (4).

  1. Hardie, D.G. (2004) J. Cell Sci. 117, 5479-87.
  2. Suzuki, A. et al. (2003) J. Biol. Chem. 278, 48-53.
  3. Suzuki, A. et al. (2004) Mol. Cell Biol. 24, 3526-35.
  4. Kusakai, G. et al. (2004) J. Exp. Clin. Cancer Res. 23, 263-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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