Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat5 (3H7) Rabbit mAb (Biotinylated) #4459

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Endogenous 90 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Stat5 (3H7) Rabbit mAb (Biotinylated) detects endogenous levels of total Stat5a and b protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a central region within human Stat5.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and UT7 cells using Stat5 (3H7) Rabbit mAb (Biotinylated).

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions and tested in-house by western blot of human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (Stat5 (3H7) Rabbit mAb #9358).

Background

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

  1. Gouilleux, F. et al. (1994) EMBO J. 13, 4361-4369.
  2. Wakao, H. et al. (1994) EMBO J. 13, 2182-2191.
  3. Okutani, Y. et al. (2001) Oncogene 20, 6643-6650.
  4. Demoulin, J.B. et al. (1999) J. Biol. Chem. 274, 25855-25861.
  5. Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.
  6. Meinke, A. et al. (1996) Mol. Cell. Biol. 16, 6937-6944.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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