Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Ran Antibody #4462

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Hm Mk B (Dm) (X) Endogenous 24 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  Dm=D. melanogaster  X=Xenopus  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Ran Antibody detects endogenous levels of total Ran protein. The antibody does not cross-react with other small GTPases of the Ras family.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with two synthetic peptides corresponding to amino acids surrounding Arg29 and Asn143 of human Ran. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, C6, COS, BAEC, and CHO cells, using Ran Antibody.

Background

Ran is a small GTPase of the Ras family that plays a central role in the spacial and temperal organization of eukaryotic cells. During interphase, Ran-GDP localizes to the cytoplasm and Ran-GTP to the nucleus. This polarized localization of Ran ensures its role in nuclear transport (1). During mitosis, Ran-GTP is chromatin associated, where it promotes spindle assembly and nuclear envolope formation (1,2). In S phase, Ran-GTP associates with and inhibits MCM helicase, ensuring precise chromosomal DNA duplication during the cell cycle (3).

  1. Quimby, B.B. and Dasso, M. (2003) Curr. Opin. Cell Biol. 15, 338-344.
  2. Hetzer, M. et al. (2002) Nat. Cell Biol. 4, 177-184.
  3. Yamaguchi, R. and Newport, J. (2003) Cell 113, 115-125.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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