Product Pathways - Cytoskeletal Signaling
Ran Antibody #4462
|4462S||100 µl (10 western blots)||---||In Stock||---|
|4462||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Hamster, Monkey, Bovine||Endogenous||24||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: D. melanogaster, Xenopus.
Specificity / Sensitivity
Ran Antibody detects endogenous levels of total Ran protein. The antibody does not cross-react with other small GTPases of the Ras family.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with two synthetic peptides corresponding to amino acids surrounding Arg29 and Asn143 of human Ran. Antibodies are purified by protein A and peptide affinity chromatography.
Ran is a small GTPase of the Ras family that plays a central role in the spacial and temperal organization of eukaryotic cells. During interphase, Ran-GDP localizes to the cytoplasm and Ran-GTP to the nucleus. This polarized localization of Ran ensures its role in nuclear transport (1). During mitosis, Ran-GTP is chromatin associated, where it promotes spindle assembly and nuclear envolope formation (1,2). In S phase, Ran-GTP associates with and inhibits MCM helicase, ensuring precise chromosomal DNA duplication during the cell cycle (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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