Cell Signaling Technology

Product Pathways - Translational Control

RagD Antibody #4470

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 50 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RagD Antibody detects endogenous levels of total RagD protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human RagD protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extract from 293 cells using RagD Antibody.

Background

The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2).

RagD is one of several genes differentially expressed in human melanoma cell lines and has been considered as a viable target for further diagnostic and therapeutic study (5).

  1. Sancak, Y. et al. (2008) Science 320, 1496-501.
  2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
  3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
  4. Wullschleger, S. et al. (2006) Cell 124, 471-84.
  5. de Wit, N.J. et al. (2005) Br J Cancer 92, 2249-61.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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