Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

HPK1 Antibody #4472

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M Endogenous 97 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HPK1 Antibody detects endogenous levels of total HPK1. The antibody does not cross-react with other members of the GCK family.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human HPK1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from WEHI231 cells, untreated or treated with staurosporine to induce HPK1 cleavage, using HPK1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat (1), U-937 (2), THP1 (3), Raw 264.7 (4), WEHI 231 (5), and Ramos cells (6), using HPK1 Antibody.

Background

Hematopoietic progenitor kinase 1(HPK1) is a member of the germinal center kinases that is predominantly expressed in hematopoietic cells. HPK1 is a key component linking T or B cell receptors to SAPK/JNK and IkappaB kinase pathways in lymphocytes (1-3). Through its proline-rich motif, HPK1 associates with multiple SH3 domain-containing adaptor proteins, including GRB2, Nck, Crk, SLP-76, and BLNK. Phosphorylation of Tyr379 by Syk is necessary for HPK1 association with BLNK and SLP-76, as well as for HPK1 activity (4,5). Caspase cleavage of HPK1 also modulates the the biological function of HPK1 (3,6,7).

  1. Liou, J. et al. (2000) Immunity 12, 399-408.
  2. Ling, S. et al. (1999) Mol. Cell. Biol. 19, 1359-1368.
  3. Arnold, R. et al. (2001) J. Biol. Chem. 276, 14675-14684.
  4. Tsuji,, . et al. (2001) J. Exp. Med. 194, 529-539.
  5. Sauer, K. et al. (2001) J. Biol. Chem. 276, 45207-45216.
  6. Chen, Y. et al. (1999) Oncogene 18, 7370-7377.
  7. Schulze-Luehrmann, J. et al. (2002) Blood 100, 954-960.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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