Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Scribble Antibody #4475

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk Endogenous 240 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Scribble Antibody detects endogenous levels of total scribble protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid residues surrounding Gly1237 of human scribble protein. Antibodies are purified by protein A and peptide affinity chromatography

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Scribble Antibody.

Background

Scribble (Scrib) was originally identified in a genetic screen in Drosophila along with cell polarity determinants Discs Large (Dlg) and Lethal giant larvae (Lgl). Drosophila mutants homozygous for these genes share similar phenotypes, including the loss of apicobasal cell polarity and neoplastic tissue overgrowth. These phenotypic similarities suggest that these three proteins function in a common pathway important for establishing and maintaining apicobasal polarity in epithelial cells (1,2). Scribble contains many leucine-rich repeats and PDZ domains important for localizing scribble to adherens junctions and basolateral regions of mammalian epithelial cells (3). Scribble reportedly binds β-catenin, APC, E-cadherin and the E6 protein from high-risk virus type of HPV through a short motif important for E6-induced cell transformation (4-8). Overexpression of scribble inhibits transformation of rodent epithelial cells by HPV E6/7 proteins (8).

  1. Bilder, D. and Perrimon, N. (2000) Nature 403, 676-80.
  2. Bilder, D. et al. (2000) Science 289, 113-6.
  3. Humbert, P.O. et al. (2008) Oncogene 27, 6888-907.
  4. Sun, Y. et al. (2009) Mol Biol Cell 20, 3390-400.
  5. Qin, Y. et al. (2005) J Cell Biol 171, 1061-71.
  6. Navarro, C. et al. (2005) Oncogene 24, 4330-9.
  7. Takizawa, S. et al. (2006) Genes Cells 11, 453-64.
  8. Nguyen, M.L. et al. (2003) J Virol 77, 6957-64.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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